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Journal: Cell reports

Article Title: D1 and D2 neurons in the nucleus accumbens enable positive and negative control over sugar intake in mice

doi: 10.1016/j.celrep.2023.112190

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit-anti-mCherry Abcam RRID: AB_2571870 TRITC-conjugated affinipure Goat anti-Rabbit IgG (H + L) Jackson ImmunoResearch Labs RRID: AB_2337932 Goat Anti-GFP antibody (FITC) Abcam RRID: AB_305635 Anti-c-Fos Rabbit polyclonal to c-Fos Abcam RRID: AB_2231989 Biotinylated Goat Anti-Rabbit IgG antibody Vector Laboratories RRID: AB_2313606 Chemicals, peptides, and recombinant proteins Sucralose Sigma PubChem Substance ID: 329769983 Rebaudioside A Sigma PubChem Substance ID: 329747271 Glucose Sigma PubChem Substance ID: 24895335 Methyl α-D-glucopyranoside Sigma PubChem Substance ID: 24897328 Denatonium Benzoate Sigma PubChem Substance ID: 57654112 Lithium chloride Sigma PubChem Substance ID: 24896459 Victoza (liraglutide) injection Novo Nordisk N/A DiI lipophiliccarbocyanine dye Sigma PubChem Substance ID: 57650110 PubChem Substance ID: Invitrogen Cat #D1817 Dextran, Tetramethylrhodamine, 10,000 MW, Lysine Fixable (Fluoro-Ruby) Critical commercial assays VECTASTAIN Elite ABC HRP Kit (Peroxidase, Standard) Vector Laboratories Cat# PK-6100 C&B Metabond Parkell SKU: S380 Experimental models: Organisms/strains Mouse: B6.FVB(Cg)-Tg(Drd1-cre)EY262Gsat/Mmucd University of California, Davis MMRRC RRID:MMRRC_030989-UCD Mouse: B6.FVB(Cg)-Tg(Drd2-cre)ER44Gsat/Mmucd University of California, Davis MMRRC RRID:MMRRC_032108-UCD Mouse: B6(Cg)-Tg(Phox2b-cre)3Jke/J The Jackson Laboratory RRID:IMSR_JAX:016223 Mouse: B6; 129S6-Gt(ROSA)26Sortm9 (CAG-tdTomato)Hze/J The Jackson Laboratory RRID:IMSR_JAX:007905 Recombinant DNA and virus strains H129 CAG-LSL-TK-tdTomato NIH Center for Neuroanatomy with Neurotropic Virus ΔTK-TT PRV CMV-EGFP NIH Center for Neuroanatomy with Neurotropic Virus Ba-152 PRV CAG-DIO-TK-GFP NIH Center for Neuroanatomy with Neurotropic Virus Ba-2017 AAV-flex-taCasp3-TEVp Addgene Cat # 45580-AAV5; RRID:Addgene_45580 AAV-EF1a-DIO-hChR2(H134R)-EYFP Addgene Cat # 20298-AAV5; RRID:Addgene_20298 Software and algorithms MATLAB R2017b MathWorks https://www.mathworks.com/products/matlab/ GraphPad Prism 8 GraphPad https://www.graphpad.com/scientific-software/prism/ Adobe Illustrator CC 2017 Adobe https://shop.adobe.com/ Open Ephys GUI Open Ephys https://open-ephys.org/software Neuroscope, NDManager and Klusters Lynn Hazan & Michaël Zugaro http://neurosuite.sourceforge.net/ KlustaKwik Ken Harris http://klustakwik.sourceforge.net/ BukaLab Asapti Labs https://asapti.com/ BioRender BioRender https://biorender.com Other Implantable Optical Fibers Doric Lenses, Canada MFC_200/240–0.22_6mm_ZF1.25(G)_FLT 32 CH Microelectrode (Electrophysiological recordings) NeuroNexus A2x16–10mm-50–500-177-A32 Micro-Renathane (tubing) Braintree SKU: MRE033 Open in a separate window KEY RESOURCES TABLE.

Techniques: Plasmid Preparation, Recombinant, Injection, Software